Can you centrifuge glass tubes?
Caution: Do not centrifuge glass tubes at forces above 2200 RCF in a horizontal head (swinging bucket) centrifuge as breakage may occur. Glass tubes may break if centrifuged above 1300 RCF in fixed angle centrifuge heads. BD Vacutainer® Plus Tubes will withstand up to 10,000 RCF in a balanced centrifuge.
What is a centrifuge tube used for?
Centrifuge tubes are used to contain liquids during centrifugation, which separates the sample into its components by rapidly rotating it around a fixed axis. Most centrifuge tubes have conical bottoms, which help collect any solid or heavier parts of the sample being centrifuged.
What tubes are used for centrifugation?
There are a variety of different tubes that can be spun in a centrifuge. Tubes with screw caps, or rubber stoppers, ones made of plastic or ones made of glass, the list goes on and on! However, the plastic Vacutainer is probably the most common tube used in a centrifuge, particularly in a hospital environment.
What plastic are centrifuge tubes made of?
polypropylene
Microcentrifuge tubes made of polypropylene (PP) and capable of holding 1.5–2.0 mL of solution have long been used for the storage and processing of biological samples, including proteins/peptides, nucleic acids, and low-molecular-weight biomol- ecules.
Do you centrifuge blue top tubes?
Allow the tube to fill until blood flow ceases to avoid dilution errors. Immediately after drawing, gently invert the tube 4 times to activate the anticoagulant and prevent clotting. Do not centrifuge.
How do you clean centrifuge tubes?
Do hand-wash tubes and bottles, using a mild detergent solution such as Beckman Solution 555™ diluted 10 to 1 with water. Rinse with distilled water, and air-dry. (Refer to the sterilization and disinfection chart below.) Do make sure all tubes and bottles are completely dry before storing them.
Why are centrifuges used?
A centrifuge is used to separate particles suspended in a liquid according to particle size and density, viscosity of the medium, and rotor speed.
How do you sterilize centrifuge tubes?
CAPP centrifuge tubes 15 mL can be sterilized by autoclaving in the temperature of 121°C for 20 minutes.
What size are centrifuge tubes?
Centrifuge tubes can be safely centrifuged to approximately 3,200 × g (50 mL size) and 3,500 × g (15 mL size) depending on the specific gravity of the solution being centrifuged and the type of rotor.
What is conical tube?
Conical tubes allow for a wider variation in the geometry across the length of the final part, making the method appropriate in more design instances. From: Encyclopedia of Materials: Science and Technology, 2001.
What color tubes do you centrifuge?
Red
Red top tubes contain no additives. Red top tubes must be allowed to clot completely (30-60 minutes) prior to centrifugation. Centrifuging the specimen yields serum. NOTE: All drug levels must be drawn in red top tubes only.