How do zebrafish induce diabetes?
(2007) introduced a primary method of inducing T2DM in zebrafish. In this method, zebrafish were alternately exposed to 2% glucose and 0% glucose solution for 30 days. Common diabetes symptoms, such as retinopathy and increased fasting blood glucose (FBS) levels were observed afterwards.
What is the diet of the zebrafish?
Zebrafish are omnivorous, primarily eating zooplankton and insects, although they can eat a variety of other foods, such as worms, small crustaceans or algae. This is one of the characteristics that makes this freshwater fish an extremely robust animal model for the laboratory.
What are zebrafish larvae?
Zebrafish larvae show a clear and distinct pattern of swimming in response to light and dark conditions, following the development of a swim bladder at 4 days post fertilization. This swimming behavior is increasingly employed in the screening of neuroactive drugs.
Why are zebrafish model organisms?
As zebrafish eggs are fertilised and develop outside the mother’s body it is an ideal model organism for studying early development. Zebrafish have a similar genetic structure to humans. They share 70 per cent of genes with us. 84 per cent of genes known to be associated with human disease have a zebrafish counterpart.
How does hyperglycemia occur?
Hyperglycemia, or high blood glucose, occurs when there is too much sugar in the blood. This happens when your body has too little insulin (the hormone that transports glucose into the blood), or if your body can’t use insulin properly. The condition is most often linked with diabetes.
What do zebrafish do?
Zebrafish have all the main organs involved in the process of metabolism and can be used to study several human metabolic disorders such as nonalcoholic fatty liver disease, type 2 diabetes mellitus, dyslipidemia, and other hepatic diseases.
How much do zebrafish weigh?
Male zebrafish weighed 0.45±0.05 g at 4 mph. After one year, average weight increased to 0.63±0.07 g; that is comparable to the average weight of 0.64±0.12 g in control fish.
What is zebrafish good for?
Why do researchers use zebrafish?
In addition to utilizing zebrafish disease models to characterize human diseases, researchers can also identify and test new drugs to treat the diseases being modeled. The ability of zebrafish to generate many embryos every time they breed makes them especially useful for high throughput drug screening.
Can blood sugar rise without diabetes?
High blood sugar is the [primary symptom that underlies diabetes], but it can also occur in people who don’t have type 1 or type 2 diabetes, either because of stress or trauma, or gradually as a result of certain chronic conditions.
Is there a zebrafish model of diet-induced obesity (Dio)?
The purpose of this study was to establish a zebrafish model of diet-induced obesity (DIO). Zebrafish were assigned into two dietary groups. One group of zebrafish was overfed with Artemia (60 mg dry weight/day/fish), a living prey consisting of a relatively high amount of fat.
Is fabp1a dysregulated in zebrafish?
One gene (fatty acid binding protein 1, FABP1) was dysregulated in all three models of mammalian obesity, but was not spotted in the zebrafish microarray. Quantitative PCR (qPCR) analysis of zebrafish fabp1a revealed a positive trend in OF8W [Additional file 7: Supplemental Figure S1].
What do you feed your zebrafish?
Zebrafish were assigned into two dietary groups. One group of zebrafish was overfed with Artemia (60 mg dry weight/day/fish), a living prey consisting of a relatively high amount of fat. The other group of zebrafish was fed with Artemia sufficient to meet their energy requirements (5 mg dry weight/day/fish).
Is agouti-related protein conserved in the zebrafish?
Song Y, Golling G, Thacker TL, Cone RD: Agouti-related protein (AGRP) is conserved and regulated by metabolic state in the zebrafish, Danio rerio. Endocrine. 2003, 22 (3): 257-265. 10.1385/ENDO:22:3:257.