What is nuclease-free water used for?
Nuclease-Free Water is used in various molecular biology applications requiring nuclease-free conditions, such as in processing DNA or RNA (i.e. PCR, cDNA synthesis, or qPCR). This product is sterile-filtered (0.2 µm), free of RNase and DNase activity, endotoxin-free, and not DEPC-treated.
What is DNase free water?
UltraPure™ DNase/RNase-Free Distilled Water is designed for use in all molecular biology applications. It is 0.1-µm membrane-filtered and tested for DNase and RNase activity. Performance and quality testing. No DNase, RNase, or protease activity detected.
Is nuclease-free water deionized?
Thermo Scientific nuclease-free water is deionized and 0.22 µm membrane-filtered nuclease-free water. It is ideal for all molecular biology applications.
How do you make nuclease-free water?
Popular Answers (1)
- Get MilliQ (reverse osmosis purified) water.
- Add 1 ml DEPC (Diethylpyrocarbonate) per 1000 ml of MilliQ or double distilled water (i.e. to a final concentration 0.1%) and mix thoroughly.
- Let the DEPC-mixed water incubate for 12 hours at 37°C.
- Autoclave DEPC-mixed water for 15 minutes.
Is Nuclease-Free Water a PCR grade?
Use of nuclease-free reagents and water, plastic consumables, and good laboratory practice, is therefore essential to avoid the degradation of samples and reagents. This PCR grade water is highly purified, DEPC treated and autoclaved to remove RNAses and DNAses, and is ideal for routine PCR work.
Does Nuclease-Free Water expire?
Expiration: 10 x 2 mL Nuclease Free Water has a shelf life of up to 2 years from analysis when stored in a sealed container in ambient conditions.
Can you drink ultrapure water?
If you do drink a glass of ultra pure water, you won’t have an immediate bad reaction, but the water will pull some minerals out of your body. As the pure water flows through you, it will attract molecules that should stay there.
Is distilled water ultrapure?
In basic, Ultrapure water would be the water which has been generated or produced through panel of technologies and gives you highest acceptable quality of water viz. 18.2mega ohm, on the other hand, Distilled water is generated by boiling the water in a still glass assembly.
Can I use distilled water for PCR?
For molecular biology experiments (PCR, cloning, protein work, etc) you should use the distilled water. For most molecular biology experiments or testing, it is best to use molecular grade ultra-purified water.
Is there a difference between distilled water and deionized water?
Distilled water has been boiled to create purified water free of contaminants and impurities like chlorine and dissolved solids. Deionized (DI) water, like distilled water, is also very pure. Where they differ is that deionized water is water that has had all the dissolved mineral particles removed from it.
What is the pH of Nuclease-Free Water?
between 5.0 and 6.5
At 22°C, Nuclease-Free Water has a pH value of between 5.0 and 6.5. It is not possible to determine the pH of highly pure water exactly.
Can you drink 100% h20?
Many people tend to imagine that purity is the ultimate indicator of the quality of water. However, 100% ultra pure water is not good for our health because water (H2O) purely comprised of hydrogen and oxygen does not provide our body with the natural electrolytes and salts that we need to survive.