What do my flow cytometry results mean?
Flow cytometry can identify the type of cells in a blood or bone marrow sample, including the types of cancer cells. It detects types of cancer cells based on either the presence or the absence of certain protein markers (antigens) on a cell’s surface.
What does CD in cd34 stand for?
cluster of differentiation
CD is an abbreviation “for cluster of differentiation”. CD molecules are cell surface markers which are very useful for the identification and characterization of leukocytes and the different subpopulations of leukocytes.
Are CD34 T cells?
Recent findings have shown that human multipotent progenitor/stem cells expressing CD34 have the capacity to differentiate into T cells when introduced into a thymic environment.
What cells are CD34+?
2.1. CD34 is a transmembrane glycoprotein expressed on early lymphohematopoietic stem cells, progenitor cells, and endothelial cells. Also, embryonic fibroblasts and some cells in fetal and adult nervous tissue are CD34-positive.
What is SSC and FSC?
The light scatter is measured by two optical detectors. One detector measures scatter along the path of the laser (1). This parameter is referred to as forward scatter (FSC). The other detector measures scatter at a ninety degree angle relative to the laser (1). This parameter is called side scatter (SSC).
What is conventional flow cytometry?
Conventional flow cytometry analysis consists of drawing a region around a population of cells (gating) and applying that region to other parameters within the experiment. This allows specific groups of cells to be selected for further analysis of other markers.
What is the final part of a flow cytometry experiment?
The final part of a flow cytometry experiment is data analysis. Traditional two parameter histogram (dot plot) gating and analysis is still being used frequently. However, the increase in number of parameters and complexity in experiments is leading to the use of newer cluster data analysis algorithms such a PCA, SPADE and tSNE.
Is flowcytometric quantitation feasible in the sentinel lymph node after cryopreservation?
Feasibility of flowcytometric quantitation of immune effector cell subsets in the sentinel lymph node of the breast after cryopreservation. J. Immunol. Methods.375: 189–195. [PubMed] [Google Scholar] 13.
What is the use of flow cytometry in TME analysis?
Last, this flow cytometry approach was used to characterize time- and dose-dependent changes to the TME following [90Y]NM600 injection in radioactive tissues requiring cryopreservation (to allow the radioactivity to decay to baseline levels to enable analysis using our core facility’s flow cytometer).