Is FLO11 a transcription factor?
FLO11 was neither up- nor down-regulated in MIG1Δ or MIG1Δ + pMIG1 relative to the wild-type, indicating FLO11 was not regulated by transcription factor MIG1 in the yeast biofilm formation process.
Do transcription factors increase gene expression?
Transcription factors are proteins that help turn specific genes “on” or “off” by binding to nearby DNA. Transcription factors that are activators boost a gene’s transcription.
What is a transcription factor in genetics?
Transcription factors are proteins involved in the process of converting, or transcribing, DNA into RNA. Transcription factors include a wide number of proteins, excluding RNA polymerase, that initiate and regulate the transcription of genes.
Why is the MIG1 protein beneficial to yeast?
MIG1 protein binds to two sites in the upstream region of SUC2, a yeast gene that is repressed by glucose. Thus, finger proteins that are similar in both amino acid sequence and DNA specificity are involved in the response of yeast to glucose, and in the mammalian early growth response.
What are Flo genes?
The FLO gene family of Saccharomyces cerevisiae includes an expressed gene, FLO11, and a set of silent, telomere-adjacent FLO genes. This gene family encodes cell-wall glycoproteins that regulate cell-cell and cell-surface adhesion.
What factors increase gene expression?
In addition to drugs and chemicals, temperature and light are external environmental factors that may influence gene expression in certain organisms.
What is the structure of hac1gene?
The HAC1gene contains 17 exons and encodes a 1,691 amino acid polypeptide with distinct domains including two ZZ-type and two TAZ-type zinc finger domains (Fig. 1, A and B). These domains have been shown in other organisms to mediate protein-protein interactions with transcription factors (Ponting et al., 1996).
How is HAC1 activated by the unfolded protein response pathway?
By the unfolded protein response pathway. Accumulation of unfolded proteins in the ER leads to activation of IRE1, which initiates splicing of the untranslated HAC1 precursor mRNA to produce the mature form.2 Publications
What is the role of Ire1 in HAC1?
Accumulation of unfolded proteins in the ER leads to activation of IRE1, which initiates splicing of the untranslated HAC1 precursor mRNA to produce the mature form.2 Publications “Endoplasmic reticulum stress-induced mRNA splicing permits synthesis of transcription factor Hac1p/Ern4p that activates the unfolded protein response.”
Which transcription factor is required for the unfolded protein-response pathway?
“Signalling from endoplasmic reticulum to nucleus: transcription factor with a basic-leucine zipper motif is required for the unfolded protein-response pathway.” Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].