What is caspase dependent apoptosis?
Apoptosis is dependent upon caspase activation leading to substrate cleavage and, ultimately, cell death. Although required for the apoptotic phenotype, it has become apparent that cells frequently die even when caspase function is blocked.
What is caspase dependent?
Release of nucleosomes and DAMPs in dying HeLa cells is dependent on caspase. To explore molecular mechanisms of cell death, the effects of cell death inhibitors were examined.
How does Taxol induce apoptosis?
Taxol-induced apoptosis is mediated by the ERK and p38 MAPK cascades in MCF7 cell and Taxol upregulates certain cell cycle proteins. Treatment of MCF7 cells with 50 ng/ml of Taxol for 1–2 days induces massive apoptosis in MCF7 cells.
What does inducing apoptosis mean?
Viral induction of apoptosis occurs when one or several cells of a living organism are infected with a virus, leading to cell death. Cell death in organisms is necessary for the normal development of cells and the cell cycle maturation. It is also important in maintaining the regular functions and activities of cells.
Does paclitaxel induce apoptosis?
Background: Compelling evidence indicates that paclitaxel kills cancer cells through the induction of apoptosis. Paclitaxel binds microtubules and causes kinetic suppression (stabilization) of microtubule dynamics.
What is the mechanism of action of Taxol?
Mechanism of action Paclitaxel-treated cells have defects in mitotic spindle assembly, chromosome segregation, and cell division. Unlike other tubulin-targeting drugs, such as colchicine, that inhibit microtubule assembly, paclitaxel stabilizes the microtubule polymer and protects it from disassembly.
What is caspase explain its function?
Caspases (cysteine-aspartic proteases, cysteine aspartases or cysteine-dependent aspartate-directed proteases) are a family of protease enzymes playing essential roles in programmed cell death. These forms of cell death are important for protecting an organism from stress signals and pathogenic attack.
How do caspases cause apoptosis?
Apoptosis is mediated by proteolytic enzymes called caspases, which trigger cell death by cleaving specific proteins in the cytoplasm and nucleus. Caspases exist in all cells as inactive precursors, or procaspases, which are usually activated by cleavage by other caspases, producing a proteolytic caspase cascade.