What is DE3 Lysogen?

What is DE3 Lysogen?

The DE3 designation means that respective strains contain the λDE3 lysogen that carries the gene for T7 RNA polymerase under control of the lacUV5 promoter. IPTG is required to maximally induce expression of the T7 RNA polymerase in order to express recombinant genes cloned downstream of a T7 promoter.

What is BL21 pLysS?

BL21(DE3)pLysS Competent Cells allow high-efficiency protein expression of any gene that is under the control of a T7 promoter and has a ribosome binding site. BL21(DE3)pLysS is lysogenic for λ-DE3, which contains the T7 bacteriophage gene I, encoding T7 RNA polymerase under the control of the lac UV5 promoter.

What are bl21-codonplus (DE3)-RIPL cells?

The BL21-CodonPlus (DE3)-RIPL cells contain extra copies of the argU, ileY,and leuWas well as the proL tRNA genes. This strain rescues expression of heterologous proteins from organisms that have either AT- or GC-rich genomes.

What is BL21 DE3 competent?

The BL21(DE3) competent cells are an all-purpose strain for high-level protein expression and easy induction. The BL21(DE3)pLysS competent cells provide tighter control of protein expression for expression of toxic proteins and are resistant to chloramphenicol.

What is BL bl21-codonplus?

BL21-CodonPlus (DE3)-RIPL Competent Cells from Agilent Technologies. Description. These cells contain extra copies of rare E. coli argU, ileY, leuW, proL tRNA genes which corrects for codon bias and dramatically improves expression of sequences from other organisms.

What is the function of BL21 cells?

The BL21(DE3)pLysS competent cells provide tighter control of protein expression for expression of toxic proteins and are resistant to chloramphenicol. When used with the CE6 bacteriophage, the BL21 cells provide the tightest control of protein expression (see BL21(DE3) Strains and Protein Toxicity).