What is kinesin heavy chain?
Kinesin heavy chain (Khc) is the plus end directed microtubule motor protein of Drosophila. Conventional kinesin (often referred to simply as kinesin) is an abundant microtubule motor protein that functions in a number of important intracellular transport processes.
How is kinesin regulated?
Mitotic kinesin motors can also be regulated by the small GTPase Ran and by cyclin-dependent kinase, Aurora and Polo-like kinases. These regulatory mechanisms ensure the proper localization and activation of kinesin motors at specific stages of the mitotic cycle.
Is kinesin a homodimer?
Conventional kinesin holoenzymes are composed of heavy and light chain homodimers. Biochemistry. 2008 Apr 15;47(15):4535-43.
Where is the kinesin motor domain?
N-terminal
The kinesin motor domain is located in the N-terminal part of most of the above proteins, with the exception of KAR3, klpA, and ncd where it is located in the C-terminal section. The kinesin motor domain contains about 330 amino acids.
What does kinesin walk on?
microtubule
Most kinesins walk towards the plus end of a microtubule, which, in most cells, entails transporting cargo such as protein and membrane components from the center of the cell towards the periphery.
What do motor proteins interact with?
Motor proteins have two essential characteristics: they interact with cytoskeletal filaments; and this interaction is modulated as the hydrolysis reaction proceeds, catalyzed by the motor domain.
Where is kinesin-1 found?
squid axoplasm
The discovery of Kinesin-1 stemmed directly from real-time observation of fast axonal transport in isolated squid axoplasm by video-enhanced light microscopy. The non-hydrolyzable ATP analog, AMP-PNP, was found to inhibit fast axonal transport and to promote tight binding of organelles to microtubules.
Do motor proteins actually walk?
Motor proteins fulfill the role of transporting large cargo about the cell to their required destinations. Kinesins are motor proteins that transport such cargo by walking unidirectionally along microtubule tracks hydrolysing one molecule of adenosine triphosphate (ATP) at each step.
What is the function of kinesin?
Kinesins are found in all eukaryotic organisms and are essential to all eukaryotic cells, involved in diverse cellular functions such as microtubule dynamics and morphogenesis, chromosome segregation, spindle formation and elongation and transport of organelles.
Where are kinesin located?
eukaryotic organisms
Kinesins are found in all eukaryotic organisms and are essential to all eukaryotic cells, involved in diverse cellular functions such as microtubule dynamics and morphogenesis, chromosome segregation, spindle formation and elongation and transport of organelles.
What is kinesin used for?
Kinesins are biological motor proteins that are ATP-dependent and function to assist cells with the transport of molecules along microtubules. Simply put, these proteins, function as highways within cells as they allow for the transport of all sorts of cellular cargo.