What is PBIN19?
Plasmid: pBin19 (ATCC staff) A vector for efficient transformation of higher plants in a binary plasmid system with pAL440. (personal communication) Medium is 1065 LB medium. NCBI gi: 520486 NCBI gi: 529322 Hosts: Agrobacterium tumefaciens, E.
What is PBIN19 Mcq?
Explanation: PBIN19 is a disarmed cloning vector which is a binary vector.
What is the size of Ti plasmid?
The T-DNA of Agrobacterium is approximately 15-20 kbp in length and will become integrated into the host plant genome upon its transfer via a process known as recombination.
What is meant by binary vector?
Abstract. A binary vector is a standard tool in the transformation of higher plants mediated by Agrobacterium tumefaciens. It is composed of the borders of T-DNA, multiple cloning sites, replication functions for Escherichia coli and A.
What is the function of onc genes in DNA Mcq?
What is the function of onc genes in TDNA? Explanation: Onc genes present in TDNA act as tumour inducing genes. Tumour inducing potential is also known as oncogenity.
In which of the plant is the crown gall disease caused Mcq?
The correct answer is option 1, i.e Crown Gall. Crown gall is a disease caused by soil-inhabiting bacterium, Agrobacterium tumefaciens. As the disease progresses, plants lose their strength and may eventually die. This disease occurs on plants including grapes, raspberries, blackberries, and roses.
What is a Ri plasmid?
Ri is the root inducing plasmid seen in Agrobacterium rhizogenes. They induce hairy root diseases in dicots. Its virulence plasmid is called pRi (Ri-Plasmid). The T-DNA of the Ri plasmid infects the plants by entering through its wounds.
Is T-DNA a transposon?
It has been proposed that processed T-DNA is first integrated into a nonessential region, such as transposons on the Agrobacterium chromosome, before that region is transferred into the plant genome (Ülker et al., 2008).
What are the uses of T-DNA insertion mutants?
Transfer DNA (T-DNA) insertion mutants are often used in forward and reverse genetics to reveal the molecular mechanisms of a particular biological process in plants. To generate T-DNA insertion mutants, T-DNA must be inserted randomly in the genome through transformation mediated by Agrobacterium tumefaciens.
What is bin19 (pBIN19)?
Binary Agrobacterium vector for plant transformation. Also known as pBIN19 or BIN19 or Bin19 or Bin 19. The 1-base overhangs produced by EcoNI may be hard to ligate. Sticky ends from different EcoNI sites may not be compatible. Sticky ends from different Bsu36I sites may not be compatible. ApaI can be used between 25°C and 37°C.
What are the most functional resistance training exercises?
Dan’s Top Ten ‘Most Functional’ Resistance Exercises 1 Squats. The Squat is quite possibly the ‘Mac-daddy’ (i.e. 2 Lunges. Using the same muscles as the squat, the lunge also replicates a primary movement pattern… 3 Deadlifts. Along with squats, the deadlift is a serious contender for the ‘Mac-daddy’… 4 Chin Ups / Pull Ups. Using all…
What are Dan Dan’s Top 10 resistance training exercises?
Dan’s Top Ten ‘Most Functional’ Resistance Exercises 1 Squats. 2 Lunges. 3 Deadlifts. 4 Chin Ups / Pull Ups. 5 Lat Pulldown. 6 Bent over Row. 7 Push Ups. 8 Bench Press. 9 Tricep Pushdown. 10 Barbell Curl.