What is silver staining method?
Silver staining is the most sensitive colorimetric method for detecting total protein. The technique involves the deposition of metallic silver onto the surface of a gel at the locations of protein bands. Silver ions (from silver nitrate in the staining reagent) interact and bind with certain protein functional groups.
How do I make silver stain?
Silver staining
- Fix the gel in fixation solution (40% ethanol, 10% acetic acid, 50% water) for 30 minutes.
- Treat the gel with protein treatment solution (20% ethanol, 5% acetic acid, 75% water, 4 mg dithiothreitol) for 30 minutes.
- Rinse the gel with 0.5% dichromate for 5 minutes.
- Wash the gel with water for 5 minutes.
When should I use silver stain?
In pathology, silver staining is the use of silver to selectively alter the appearance of a target in microscopy of histological sections; in temperature gradient gel electrophoresis; and in polyacrylamide gels.
How sensitive is silver staining?
Silver staining Kits with optimized protocols are robust and easy to use, detecting less than 0.5 ng of protein in typical gels. Silver stains use either glutaraldehyde or formaldehyde as the enhancer.
Is silver staining toxic?
Current silver staining methods have had the disadvantages of long development time (12-24 hours), unstable solutions, and very toxic chemicals. Sigma’s method is 3.5 hours from fixed to stained gel. Sigma’s solutions are less toxic and stable as shipped.
What is blue silver staining?
A modified Neuhoff’s colloidal Coomassie Blue G-250 stain is reported, dubbed “blue silver” on account of its considerably higher sensitivity, approaching the one of conventional silver staining.
What is maneval’s stain?
In Maneval’s capsule staining method, the basic stain that interacts with the bacterial cell is acid fuchsin. Acid fuchsin is present in the formulation of Maneval’s solution. The counterstaining is provided by Congo red.