What is the mechanism of action of urokinase?
Because urokinase is a protein, it is expected to be metabolized by proteases to smaller proteins and amino acids. Urokinase is eliminated in the bile and urine. Urokinase has a half life of 12.6±6.2 minutes….Pharmacology.
| Target | Actions | Organism |
|---|---|---|
| AUrokinase plasminogen activator surface receptor | inducer modulator | Humans |
What is the function of urokinase?
Urokinase (UK) is a serine protease, which is one of biological plasminogen activators. It is involved in a number of biological functions including fibrinolysis, embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing. It can be obtained from human urine or kidney cell culture.
What is the function of urokinase plasminogen activator as it relates to tumors?
Its main function is focusing of urokinase (uPA) proteolytic activity, responsible for degradation of extracellular matrix (ECM) components, on the cell surface1. uPAR expression is increased in many human cancers and correlates with a poor prognosis and early invasion and metastasis2.
Is uPA secreted?
Human urokinase-type plasminogen activator (uPA) is a serine protease that converts plasminogen to plasmin. It is produced and secreted by a variety of different human cells in vivo and in vitro.
What does urokinase mean?
Definition of urokinase : an enzyme that is produced by the kidney and found in urine, that activates plasminogen, and that is used therapeutically to dissolve blood clots (as in the heart)
Is urokinase an anticoagulant?
Consequently, the administration of urokinase as a sole anticoagulant results in activation of coagulation and fibrinolysis, and, as a result, induces disseminated intravascular coagulation.
When do you use urokinase?
Kinlytic™ (urokinase injection) is indicated in adults:
- For the lysis of acute massive pulmonary emboli, defined as obstruction of blood flow to a lobe or multiple segments.
- For the lysis of pulmonary emboli accompanied by unstable hemodynamics, i.e., failure to maintain blood pressure without supportive measures.
What is urokinase made from?
Commercially available urokinase, the low-molecular-weight form (32 400 Da), is produced from cultured human neonatal kidney cells. It is a two-polypeptide chain serine protease, containing 411 amino acid residues4,35: an A chain of 2000 Da is linked by a sulfhydryl bond to a B chain of 30 400 Da.
Is urokinase made from urine?
Urokinase is an endogenous activator, which is produced by kidney cells and can be recovered from urine. It directly activates plasminogen and is now manufactured with use of recombinant DNA technology.
Can urokinase be refrigerated?
Refrigerate Kinlytic™ (urokinase injection) powder at 2° to 8°C (36° to 46°F) (See USP).
Who discovered urokinase?
It was first identified in the urine in 1947 by MacFarlane and Pilling who reported on the fibrinolytic activity of a novel “unnamed” protein (24). Half a decade later, Sobel and colleagues named this “unnamed” protein as “urokinase” (25).
Is urokinase a drug?
Urokinase is a thrombolytic (THROM-bo-LIT-ik) drug, sometimes called a “clot-busting” drug. It helps your body produce a substance that dissolves unwanted blood clots. Urokinase is used to treat blood clots in the lungs. Urokinase may also be used for purposes not listed in this medication guide.
What is the urokinase receptor uPAR?
The urokinase receptor (uPAR; CD-87) is a 50–60 kDa heavily glycosylated cell surface receptor for the serine protease urokinase (uPA; Kd=1 nM). uPAR is linked to the exoplasmic leaflet of the plasma membrane through a C-terminal glycosylphosphatidylinositol (GPI) chain ( Figure 2 ).
What are the binding partners of the uPAR receptor?
Its immediate binding partners are the serine proteinase urokinase (uPA), and vitronectin (VTN), a component of the extracellular matrix. uPA and VTN b … The urokinase receptor (uPAR) is a GPI-anchored cell surface receptor that is at the center of an intricate network of protein-protein interactions.
What is the role of UPA and Upar in cancer progression?
In cancer, increased levels of the tumor-associated serine protease uPA (urokinase-type plasminogen activator) and its receptor uPAR (CD87) are linked to tumor progression, metastasis, and shortened survival in patients afflicted with this disease. Strong clinical and experimental evidence has accum …
What does Upar stand for?
Urokinase plasminogen activator receptor (uPAR) is a glycosylphosphatidylinositol-anchored glycoprotein and cell surface receptor specific to the urokinase plasminogen activator (uPA) and is a promoter of plasmin activation. uPAR consists of three homologous domains, denoted by I, II, and III [147].