Which cell lines used in biologics?
The most frequently used mammalian system is the CHO cell line, which is used in the manufacture of >70% of currently approved recombinant proteins (Butler & Spearman, 2014). This cell line has demonstrated several major advantages.
What is recombinant cell line?
Definition. A cell line into which recombinant DNA has been introduced, either stably or transiently.
Where do mammalian cell lines come from?
Classically they are obtained from embryos – the so called embryonic stem cells. Others can be extracted from certain tissues, e.g. the bone marrow contains blood stem cells. These kinds of cells are called “tissue-specific” or “adult” stem cells.
How are cell lines developed?
The simplest way to create a new cell line is to modify an existing one, a common strategy when an established line already comes close to meeting the requirements. Cells optimized to grow particular viruses or maximize recombinant protein production often come from such modifications.
Why is cell culture important for biological medical research?
Experimental model systems in basic and medical sciences. Cell culture offers certain advantages over the environmental and biological variability of other models. In addition, the use of genetically defined and characterized cell lines can simplify the analysis of experimental data.
What do mammalian cells produce?
Mammalian cells can be made to produce vaccines through viral infection, and therapeutic proteins through genetic engineering. Many of these medicines are necessary for patients who either lack the normal form of a protein or cannot produce it in sufficient quantity.
Why is mammalian cell culture important?
Mammalian cell culture is an important tool for research, clinical, and pharmaceutical applications. Cells isolated from animal tissues can be expanded in culture to study cell biology and disease or used for the production of antibodies, proteins, and vaccines.
What is mammalian tissue?
Mammalian cells are cells that are derived or isolated from tissue of a mammal. In this experiment, students are introduced to four mammalian cell types: fibroblasts, epithelial cells, lymphocytes and macrophages. Lymphocytes are found within the blood.